The use of herbs against neglected diseases: Evaluation of leishmanicidal and trypanocidal activity of Mart.
Journal:
Saudi journal of biological sciences
Published Date:
Mar 7, 2015
Abstract
The evaluation of the leishmanicidal and trypanocidal activity of the hydroalcoholic extract of the bark of Mart. (EHCSR) was carried out to find an alternative treatment for parasitic diseases. EHCSR was prepared and used at four different concentrations (1000, 500, 250, 125 μg/mL) in assays for activity against Leishmania promastigotes using the species and and for trypanocidal activity using the epimastigotes of . We also tested EHCSR for cytotoxicity against adhered cultured Murine J774 fibroblasts. The tests were performed in triplicate, and the percent mortality of parasites, IC and percent toxicity were determined. With regard to anti-leishmania activity against , there was a mean mortality of 45% at all concentrations, and against , a substantial effect was seen at 1000 μg/mL with 56.38% mortality, where the IC values were 1338.76 and 987.35 μg/mL, respectively. Trypanocidal activity was notably high at 1000 μg/mL extract with 82.31% mortality of epimastigotes. Cytotoxicity at the highest extract concentrations of 500 and 1000 μg/mL was respectively 75.12% and 94.14%, with IC = 190.24 μg/mL. Despite that the extract has anti-parasitic activity, its substantial cytotoxicity against fibroblasts cells makes its systemic use nonviable as a therapeutic alternative.
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