Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology.

Journal: Jundishapur journal of microbiology
Published Date: Dec 26, 2015

Abstract

BACKGROUND: Escherichia coli phytase is an acidic histidine phytase with great specific activity. Pichia pastoris is a powerful system for the heterologous expression of active and soluble proteins which can express recombinant proteins in high cell density fermenter without loss of product yield and efficiently secrete heterologous proteins into the media. Recombinant protein expression is influenced by expression conditions such as temperature, concentration of inducer, and pH. By optimization, the yield of expressed proteins can be increase. Response surface methodology (RSM) has been widely used for the optimization and studying of different parameters in biotechnological processes.

Authors

  • Ali Akbarzadeh
    Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran.
  • Ehsan Dehnavi
    Gene Transfer Pioneers Research Group, Shahid Beheshti University, Tehran, IR Iran.
  • Mojtaba Aghaeepoor
    Gene Transfer Pioneers Research Group, Shahid Beheshti University, Tehran, IR Iran; Semnan Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, IR Iran.
  • Jafar Amani
    Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

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