Determination of Four Major Saponins in Skin and Endosperm of Seeds of Horse Chestnut (Aesculus Hippocastanum L.) Using High Performance Liquid Chromatography with Positive Confirmation by Thin Layer Chromatography.

Journal: Advanced pharmaceutical bulletin
Published Date: Nov 30, 2015

Abstract

PURPOSE: To separate and quantify four major saponins in the extracts of the skin and the endosperm of seeds of horse chestnut (Aesculus hippocastanum L.) using ultrasonic solvent extraction followed by a high performance liquid chromatography-diode array detector (HPLC-DAD) with positive confirmation by thin layer chromatography (TLC).

Authors

  • Zead Helmi Mahmoud Abudayeh
    Faculty of Pharmacy, Isra University, 11622 Amman, Jordan.
  • Khaldun Mohammad Al Azzam
    Department of Pharmaceutical Chemistry, Pharmacy Program, Batterjee Medical College for Sciences and Technology (BMC), 21442 Jeddah, Kingdom of Saudi Arabia.
  • Ahmad Naddaf
    Faculty of Pharmacy, Isra University, 11622 Amman, Jordan.
  • Uliana Vladimirovna Karpiuk
    Department of Pharmacognosy and Botany, National Medical University is the Name of O.O.Bogomolets, Ukraine.
  • Viktoria Sergeevna Kislichenko
    National University of Pharmacy, Kharkiv, Ukraine.

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