A comprehensive update on the application of high-throughput fluorescence imaging for novel drug discovery.

Journal: Expert opinion on drug discovery
Published Date: May 5, 2025

Abstract

INTRODUCTION: High-throughput fluorescence imaging (HTFI) is revolutionizing drug discovery by enabling rapid and precise detection of biological targets and cellular processes. Recent advances in fluorescence imaging technologies now provide unprecedented sensitivity, resolution, and throughput. Integration of artificial intelligence (AI) and machine learning (ML) into HTFI workflows significantly enhances data processing, aiding in hit identification, pattern recognition, and mechanistic understanding.

Authors

  • Michael Ronzetti
    National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.
  • Anton Simeonov
    National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.

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