Study on the effect and mechanism of PM on the expression of alzheimer's-like lesions related proteins in SH-SY5Y cells.

Journal: Toxicology letters
Published Date: May 9, 2025

Abstract

Fine particulate matter (PM) is recognized as one of the most harmful environmental pollutants to human health. Current research indicates that PM exhibits neurotoxic effects, though the specific mechanisms remain unclear. In this study, SH-SY5Y cells were exposed to PM (100μg/mL for 24h) to observe its effects on the expression of Alzheimer's disease (AD)-related proteins and explore the possible mechanisms of central nervous system injury caused by PM. Based on bioinformatics results, the study employed a PI3K inhibitor (LY294002, 10 μmol/L for 1h) and a reactive oxygen species (ROS) inhibitor, N-acetyl-L-cysteine (NAC, 5 nmol/L for 1h), as interventions. The results demonstrated that PM exposure significantly intensified oxidative stress in SH-SY5Y cells, upregulated the expression of inflammatory factors, and increased apoptosis. Additionally, exposure to PM led to elevated levels of AD-related pathological proteins, including amyloid-β (Aβ), and promoted Tau phosphorylation, further indicating its potential neurotoxic effects. Furthermore, the ROS/PI3K/Akt/GSK-3β pathway was found to play a key role in these processes. This research provides a basis for understanding the impact of PM on Alzheimer's disease patients and offers recommendations for the prevention of haze-related health risks, as well as for risk management by relevant governmental departments.

Authors

  • Huiwen Kang
    Department of Occupational and Environmental Health, Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, School of Public Health, North China University of Science and Technology, Tangshan, 063210, PR China.
  • Si Chenyang
    Department of Occupational and Environmental Health, Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, School of Public Health, North China University of Science and Technology, Tangshan, 063210, PR China.
  • Xuan Shang
    Department of Occupational and Environmental Health, Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, School of Public Health, North China University of Science and Technology, Tangshan, 063210, PR China.
  • Qian Song
    Department of Occupational and Environmental Health, School of Public Health, Qingdao University, Qingdao 266071, China.
  • Wenli Zhang
    Comprehensive Testing Center, North China University of Science and Technology, Tangshan, 063210, PR China.
  • Jinlong Li
    Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
  • Shoufang Jiang
    Department of Occupational and Environmental Health, Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, School of Public Health, North China University of Science and Technology, Tangshan, 063210, PR China. Electronic address: jiangshoufang@163.com.

Keywords

No keywords available for this article.

External Resources

Popular Topics
Recent Journals