Structural, functional and antioxidant characteristics of enzymatic hydrolysates from Cantharellus cibarius protein.

Journal: Food chemistry
Published Date: May 7, 2025

Abstract

This study first explored the antioxidant capacity, structural characterization, and functional characteristics of Cantharellus cibarius Fr. protein (CCP) and its enzymatic hydrolysates (CCPHs). CCP was extracted via ultrasonic-assisted alkaline extraction/acid precipitation, and six CCPHs were generated using single (alcalase, neutrase, flavourzyme, bromelain) or two step enzymatic hydrolysis (alcalase-flavourzyme, flavourzyme-alcalase). FA-CCPH showed the highest hydrolysis degree (63.39 ± 0.71 %), optimal DPPH scavenging (IC = 1.324 mg/mL) and superior ferric-reducing power (1.19 ± 0.01 at 5 mg/mL). AF-CCPH displayed the most effective ABTS radical scavenging activity (IC = 0.328 mg/mL). Enzymatic hydrolysis concentrated particle size distribution, disrupted tertiary structures, reduced hydrophobicity, and altered secondary structure ratios of CCPHs. CCPHs displayed porous and fractured surfaces compared to CCP. Distinct functional properties were observed among hydrolysates. These findings support the potential application of CCPHs as antioxidant ingredients in functional foods.

Authors

  • Yunxiang Que
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Xingrui Liu
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Pinyao Liu
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Wenqi Liu
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Haipeng Zhang
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Chengguang He
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Huining Dai
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Linna Du
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Hongxia Ma
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
  • Lili Guan
    College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China. Electronic address: llguan@jlau.edu.cn.

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