A MOF-818-based nanozyme-linked aptasensor for microcystin-LR detection in edible algae powder.

Journal: Talanta
Published Date: May 2, 2025

Abstract

The development of simple, rapid, and sensitive detection methods for microcystin-leucine-arginine (MC-LR) is crucial for ensuring food safety. A novel nanozyme-linked colorimetric aptasensor was developed using metal-organic framework (MOF) material MOF-818. The aptamer and MOF-818 with complementary DNA (cDNA) on its surface (MOF-818/cDNA) served as bioreceptors and signal reporters, respectively. Without MC-LR, MOF-818/cDNA bound to the aptamer, catalyzing the oxidation of 3,5-Di-tert-butylcatechol (3,5-DTBC) and producing yellow-colored products. While in the presence of MC-LR, it bound to the aptamers competitively, inhibited MOF-818/cDNA capture and reduced catalytic activity, enabling colorimetric quantification. The method achieved a linear detection range of 1-200 ng/mL and a limit of detection of 0.849 ng/mL. It successfully quantified MC-LR in spiked lake water and tap water, as well as MC-LR-contaminated edible algae (Spirulina platensis and Chlorella pyrenoidosa) powders, with recovery rates ranging from 81.45 % to 136.67 %. This research provided a cost-effective, efficient and portable method for MC-LR screening in food and environmental samples.

Authors

  • Junlan Yu
    Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
  • Wenjie Li
    Yunnan Agricultural University, Kunming, China.
  • Jiachen Zhao
    Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
  • Guijie Hao
    Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Huzhou Key Laboratory of Aquatic Product Quality Improvement and Processing Technology, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, China.
  • Zhe Feng
    Department of Cardiovascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
  • Fangkai Han
    School of Biological and Food Engineering, Suzhou University, Suzhou, 234000, China.
  • Jiahua Wang
    Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
  • Xiaodan Liu
    Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
  • Fuwei Pi
    Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China; State Key Laboratory of Food Science and Resources, School of Food Science, Jiangnan University, Wuxi, 214122, China.
  • Huang Dai
    Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. Electronic address: huangdai9@126.com.
  • Xin Liu
    Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences, Weifang, Shandong, China.
  • Yafang Shen
    Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Huzhou Key Laboratory of Aquatic Product Quality Improvement and Processing Technology, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, China. Electronic address: shenyafang1994@126.com.

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