Shortcoming of serum B-cell maturation antigen measurement by enzyme-linked immunosorbent assay in one laboratory's experience: Unsatisfactory assay reproducibility.

Journal: Clinical biochemistry
Published Date: May 7, 2025

Abstract

INTRODUCTION: Serum protein electrophoresis and serum free light chain (SFLC) assays are standard methods for monitoring patients with multiple myeloma (MM). However, patients with non-secretory MM often require invasive bone marrow biopsies to monitor treatment response and disease progression. Recently, serum soluble B-cell maturation antigen (sBCMA) has been proposed as an alternative biomarker for monitoring of MM, including non-secretory disease. We aimed to optimize the performance of and validate a serum sBCMA enzyme-linked immunosorbent assay (ELISA) from R&D Systems for research and eventual clinical use.

Authors

  • Ian Cheong
    Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada.
  • Christopher Liang
    Faculty of Science, Western University, London, ON, Canada.
  • Vipin Bhayana
    Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada; Department of Pathology and Laboratory Medicine, London Health Sciences Centre and St. Joseph's Health Care London, London, ON, Canada.
  • Ivan Stevic
    Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada; Department of Pathology and Laboratory Medicine, London Health Sciences Centre and St. Joseph's Health Care London, London, ON, Canada.
  • Martha Louzada
    Division of Hematology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada.
  • Ian Chin-Yee
    Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada; Department of Pathology and Laboratory Medicine, London Health Sciences Centre and St. Joseph's Health Care London, London, ON, Canada; Division of Hematology, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada.
  • Angela C Rutledge
    Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, Western University, London, ON, Canada; Department of Pathology and Laboratory Medicine, London Health Sciences Centre and St. Joseph's Health Care London, London, ON, Canada. Electronic address: Angela.Rutledge@lhsc.on.ca.

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