Use of polyethylene glycol precipitation and ultracentrifugation to enhance the sensitivity of hepatitis B virus DNA detection.

Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Published Date: May 3, 2025

Abstract

BACKGROUND: Sensitive molecular detection of hepatitis B virus (HBV) DNA is crucial for diagnosing and managing occult hepatitis. To improve the sensitivity of HBV DNA detection, we compared the effectiveness of polyethylene glycol (PEG) precipitation and ultracentrifugation to concentrate DNA prior to extraction.

Authors

  • Michael X Fu
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom. Electronic address: michael.fu@ndm.ox.ac.uk.
  • Osmany Larralde
    National Microbiology Reference Unit, Scottish National Blood Transfusion Service, Edinburgh, United Kingdom.
  • Richard Mayne
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
  • Kai Kean
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
  • Kaitlin Reid
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
  • Monique Andersson
    Department of Microbiology, Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom; Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom.
  • Tanya Golubchik
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom; Sydney Infectious Diseases Institute, Faculty of Medicine and Health, University of Sydney, Sydney, Australia.
  • Jane A McKeating
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
  • Lisa Jarvis
    National Microbiology Reference Unit, Scottish National Blood Transfusion Service, Edinburgh, United Kingdom.
  • William L Irving
    NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust, University of Nottingham, Nottingham, United Kingdom.
  • Peter Simmonds
    Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
  • Heli Harvala
    Radcliffe Department of Medicine, University of Oxford, Oxford, United Kingdom; Microbiology Services, NHS Blood and Transplant, Colindale, United Kingdom; Institute of Biomedicine, University of Turku and University Hospital of Turku, Turku, Finland. Electronic address: heli.harvala@utu.fi.

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