Phage Ge15, NRG-P0073: Genomic Characterization and Host Range Analysis Against the ECOR Reference Library.

Journal: PHAGE (New Rochelle, N.Y.)
Published Date: Jun 4, 2025

Abstract

BACKGROUND: Genomic sequencing and annotation, morphological characterization, and host range analyses of bacteriophage (phage) isolates are crucial to understanding each phage's unique set of properties and how they can be utilized as effective tools in medicine, environmental monitoring, biotechnology, and agriculture. In this study, we present the fully annotated genome of viral isolate phage Ge15 (GenBank Accession No. PP359696.1), taxonomically identified as unclassified Tequatrovirus, and deposited into our strain collection as sample NRG-P0073. A host range analysis was performed against all 72 isolates of the Reference (ECOR) library and a selection of K-12 single-gene knockouts from the Keio collection in an effort to identify the receptor-binding protein.

Authors

  • Ranee K Anderson
    Department of Food Science, Cornell University, Ithaca, NY, USA.
  • Shijie Qin
    Department of Food Science, Cornell University, Ithaca, NY, USA.
  • Rachel M Carson
    Department of Food Science, Cornell University, Ithaca, NY, USA.
  • Sam R Nugen
    Department of Food Science and Technology, Cornell University, Ithaca, New York 14853, United States.

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