Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems.

Journal: HardwareX
Published Date: Mar 19, 2025

Abstract

Computerized microscopes improve repeatability, throughput, antisepsis, data analysis and data sharing in the biological laboratory, but these machines are cost-prohibitive in most academic environments. This is a barrier into collecting the large and consistent datasets required for machine learning analyses of microscopy data. We demonstrate hardware modifications and software to bring the features of modern computerized microscopes to decades-old legacy laboratory inverted microscopes. We demonstrate automation of X-Y positioning, focus stacking, image acquisition and image storage.

Authors

  • Drew Ehrlich
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Yohei Rosen
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • David F Parks
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Kivilcim Doganyigit
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Ryan Fenimore
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Samira Vera-Choqqueccota
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Sebastian Hernandez
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Anna Toledo
    Department of Performance, Play and Design, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • David Haussler
    Genomics Institute, University of California, Santa Cruz, Santa Cruz, California, United States of America.
  • Sri Kurniawan
    UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
  • Mircea Teodorescu
    Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, California, United States of America.

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