An MOF-Enhanced Anti-Fouling Immunoprobe Platform for Efficient Direct Screening of Pancreatic Cancer.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published Date: Jul 1, 2025

Abstract

Monitoring biomarkers offers insights for early disease (e.g., cancer, chronic diseases) screening, treatment guidance and response evaluation. To tackle challenges in precise biomarker detection in complex blood samples, we propose a metal-organic framework (MOF)-enhanced anti-fouling immunoprobe (MAIP). This sensing platform integrates several key features, including an anti-fouling surface, fully oriented antibody immobilization, and an enhanced electron transfer layer. This platform demonstrates remarkable sensitivity and specificity, capable of detecting trace concentrations of cancer markers, ranging from pg/mL to ng/mL. Comprehensive analysis of clinical serum samples, combined with machine learning (ML) evaluations, underscores the platform's reliability and effectiveness in cancer biomarker detection. Using a combination of three biomarkers, the algorithm demonstrated satisfactory classification performance with an accuracy of 100% on the test set across 64 serum samples. Moreover, when evaluated using 10-fold cross-validation, the optimized model maintained a robust accuracy of 0.955 ± 0.09, reinforcing its strong stability and good generalization ability across different data partitions. The MAIP-based platform developed here offers swift analysis, high accuracy, and a simplified testing process. This makes it a promising approach for directly profiling cancer markers from blood, thereby facilitating timely and effective patient care.

Authors

  • Wenjuan Yang
    Department of General Practice, Community Health Service Center of Guali Town of Xiaoshan, Hangzhou, China. Electronic address: ay852832318@163.com.
  • Shujuan Mao
    Materials Genome Institute, Shanghai Engineering Research Center for Integrated Circuits and Advanced Display Materials, Shanghai University, Shanghai, 200444, China.
  • Hao Zhu
    State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology Wuhan 430070 PR China chang@whut.edu.cn suntl@whut.edu.cn.
  • Handong Yao
    Materials Genome Institute, Shanghai Engineering Research Center for Integrated Circuits and Advanced Display Materials, Shanghai University, Shanghai, 200444, China.
  • Wei Jin
    Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR China; Institute of Cardiovascular Diseases, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR China. Electronic address: jinwei1125@126.com.
  • Lingyan Feng
    Materials Genome Institute, Shanghai Engineering Research Center for Integrated Circuits and Advanced Display Materials, and Shanghai Engineering Research Center of Organ Repair, Shanghai University, Shanghai, 200444, China.
  • Xinghua Gao
    NHC Key Laboratory of Immunodermatology (China Medical University), Ministry of Education Key Laboratory of Immunodermatology (China Medical University), Department of Dermatology The First Hospital of China Medical University, Shenyang, China.
  • Xuefeng Wang
    Department of Advanced Manufacturing and Robotics, College of Engineering, Peking University, Beijing 100871, China.
  • Wei Chen
    Department of Urology, Zigong Fourth People's Hospital, Sichuan, China.
  • Min Tu
  • Yuan Zhang
    Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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