Side-to-side characterisation of cellular content, soluble factors and in vitro potential on chondrocytes for bone marrow aspirate concentrate and adipose-derived stromal vascular fraction.

Journal: Journal of experimental orthopaedics
Published Date:

Abstract

PURPOSE: Orthobiologics gained popularity for the treatment of musculoskeletal pathologies, including osteoarthritis (OA). Bone marrow aspirate concentrate (BMAC) and adipose-derived stromal vascular fraction (SVF) were reported to reduce OA symptoms, contributing to the restoration of joint homoeostasis. Variability in production protocols and lack of extensive characterisation hinder a clear indication for the choice of a product over the other. The purpose of this study was to characterise side-by-side BMAC and SVF obtained with the same family of devices, by assessing cell immunophenotype, release of soluble factors and their ability to reduce inflammation in a pathologic in vitro chondrocyte model.

Authors

  • Enrico Ragni
    IRCCS Ospedale Galeazzi-Sant'Ambrogio, Laboratorio di Biotecnologie Applicate all'Ortopedia Milano Italy.
  • Michela Taiana
    IRCCS Ospedale Galeazzi-Sant'Ambrogio, Laboratorio di Biotecnologie Applicate all'Ortopedia Milano Italy.
  • Caterina Visconte
    IRCCS Ospedale Galeazzi-Sant'Ambrogio, Laboratorio di Biotecnologie Applicate all'Ortopedia Milano Italy.
  • Elizaveta Kon
    IRCCS Humanitas Research Hospital Rozzano Italy.
  • Simona Landoni
    IRCCS Ospedale Galeazzi-Sant'Ambrogio, Laboratorio di Biotecnologie Applicate all'Ortopedia Milano Italy.
  • Cecilia Colombo
    IRCCS Ospedale Galeazzi-Sant'Ambrogio, Laboratorio di Biotecnologie Applicate all'Ortopedia Milano Italy.
  • Laura Mangiavini
    IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
  • Giuseppe Peretti
    Department of Biomedical Sciences for Health University of Milan Milan Italy.
  • Laura de Girolamo
    IRCCS Ospedale Galeazzi-Sant'Ambrogio, Laboratorio di Biotecnologie Applicate all'Ortopedia Milano Italy.

Keywords

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