Self-Evolving Discovery of Carrier Biomaterials with Ultra-Low Nonspecific Protein Adsorption for Single Cell Analysis.

Journal: Advanced materials (Deerfield Beach, Fla.)
Published Date: Jul 2, 2025

Abstract

Carrier biomaterials used in single-cell analysis face a bottleneck in protein detection sensitivity, primarily attributed to elevated false positives caused by nonspecific protein adsorption. Toward carrier biomaterials with ultra-low nonspecific protein adsorption, a self-evolving discovery is developed to address the challenge of high-dimensional parameter spaces. Automation across nine self-developed or modified workstations is integrated to achieve a "can-do" capability, and develop a synergy-enhanced Bayesian optimization algorithm as the artificial intelligence brain to enable a "can-think" capability for small-data problems inherent to time-consuming biological experiments, thereby establishing a self-evolving discovery for carrier biomaterials. Through this approach, carrier biomaterials with an ultra-low nonspecific protein adsorption index of 0.2537 are successfully discovered, representing an over 80% decrease, while achieving a 10 000-fold reduction in experiment workload. Furthermore, the discovered biomaterials are fabricated into microfluidic-used carriers for protein-analysis applications, showing a 9-fold enhancement in detection sensitivity compared to conventional carriers. This is the very demonstration of a self-evolving discovery for carrier biomaterials, paving the way for advancements in single-cell protein analysis and further its integration with genomics and transcriptomics.

Authors

  • Songtao Hu
    State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Wenhui Lu
    Shanghai Key Laboratory of Forensic Medicine and Key Laboratory of Forensic Science, Ministry of Justice, Shanghai 200063, PR China; Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, Shandong 250014, PR China. Electronic address: whlu@sdupsl.edu.cn.
  • Xijia Ding
    State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Yingying Xue
    Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, Guangzhou, 510005, China.
  • Congcong Liu
    Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, School of Life Science and Technology, University of Electronic Science and Technology, Chengdu, China.
  • Tian Xie
    Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, School of Medicine, Hangzhou Normal University, Hangzhou, China.
  • Yinjun Deng
    State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Haoran Li
    School of Quality and Technical Supervision, Hebei University, Baoding, Hebei 071002, P.R.China.
  • Zhuocheng Gong
    Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, Guangzhou, 510005, China.
  • Yanming Xia
    Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, Guangzhou, 510005, China.
  • Peishen He
    Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, Guangzhou, 510005, China.
  • Lingliao Zeng
    Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, Guangzhou, 510005, China.
  • Zhong Wang
    Department of Intensive Care Unit, The First Hospital of China Medical University, Shenyang, Liaoning, China.
  • Jian Jin
    Department of Agricultural and Biological Engineering, Purdue University, 225 S. University St., West Lafayette, IN 47907, USA.
  • Zhi Luo
    Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.
  • Xi Shi
    State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Zhike Peng
    State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Tao Xu
    Department of Urology, Peking University People's Hospital, Beijing, China.
  • Xiaobao Cao
    Guangzhou National Laboratory, No. 9 XingDaoHuanBei Road, Guangzhou International Bio Island, Guangzhou, Guangzhou, 510005, China.

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