Nitrogen limitation causes a seismic shift in redox state and phosphorylation of proteins implicated in carbon flux and lipidome remodeling in Rhodotorula toruloides.

Journal: Biotechnology for biofuels and bioproducts
Published Date: Jul 21, 2025

Abstract

BACKGROUND: Oleaginous yeast are prodigious producers of oleochemicals, offering alternative and secure sources for applications in foodstuff, skincare, biofuels, and bioplastics. Nitrogen starvation is the primary strategy used to induce oil accumulation in oleaginous yeast as part of a global stress response. While research has demonstrated that post-translational modifications (PTMs), including phosphorylation and protein cysteine thiol oxidation (redox PTMs), are involved in signaling pathways that regulate stress responses in metazoa and algae, their role in oleaginous yeast remain understudied and unexplored.

Authors

  • Austin Gluth
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Jeffrey J Czajka
    Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
  • Xiaolu Li
    Department of Rehabilitation Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China.
  • Kent J Bloodsworth
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
  • Josie G Eder
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
  • Jennifer E Kyle
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
  • Rosalie K Chu
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Bin Yang
    School of Control Science and Engineering, Dalian University of Technology, Dalian 116024, PR China. Electronic address: yangbin@dlut.edu.cn.
  • Wei-Jun Qian
    Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Pavlo Bohutskyi
    Biological Sciences Division, Pacific Northwest National Laboratory, 3300 Stevens Dr., Richland, WA 99354, USA. Electronic address: pavlo.bohutskyi@pnnl.gov.
  • Tong Zhang
    Beijing University of Chinese Medicine, Beijing, China.

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