Direction and modality of transcription changes caused by TAD boundary disruption in Slc29a3/Unc5b locus depends on tissue-specific epigenetic context.

Journal: Epigenetics & chromatin
Published Date: Aug 12, 2025

Abstract

BACKGROUND: Topologically associating domains (TADs) are believed to play a role in the regulation of gene expression by constraining or guiding interactions between the regulatory elements. While the impact of TAD perturbations is typically studied in developmental genes with highly cell-type-specific expression patterns, this study examines genes with broad expression profiles separated by a strong insulator boundary. We focused on the mouse Slc29a3/Unc5b locus, which encompasses two distinct TADs containing ubiquitously expressed and essential for viability genes. We disrupted the CTCF-boundary between these TADs and analyzed the resulting changes in gene expression.

Authors

  • Paul Salnikov
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Polina Belokopytova
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Alexandra Yan
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Emil Viesná
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Alexey Korablev
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Irina Serova
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Varvara Lukyanchikova
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Yana Stepanchuk
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Nikita Torgunakov
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Savelii Tikhomirov
    Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Veniamin Fishman
    AIRI, Moscow, 121170, Russia.

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