Simple and Rapid HPLC-ICP-MS Method for the Simultaneous Determination of Cr(III) and Cr(VI) by Combining a 2,6-Pyridinedicarboxylic Acid Pre-Complexation Treatment.
Journal:
Mass spectrometry (Tokyo, Japan)
Published Date:
Dec 18, 2024
Abstract
A simple and rapid analytical method was developed for the simultaneous determination of two chromium species, Cr(III) and Cr(VI), in the environmental waters by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). This study incorporated a chelating pretreatment with 2,6-pyridinedicarboxylic acid (PDCA) to convert Cr(III) species into a stable Cr(III)-PDCA anion complex, which was then separated from Cr(VI) oxyanion using an anion exchange column. Building on the fundamental analytical approach proposed by Shigeta . (doi: 10.2116/analsci.18P012), the mobile phase was optimized to ensure stability for ICP-MS detection, avoiding nonvolatile salts. Chromium species and chloride ions were effectively separated within 6 minutes at a flow rate of 0.6 mL min with the optimized mobile phase, which consisted of 50 mmol L ammonium acetate (pH 6.80) and 2 mmol L PDCA. The detection limits were 0.18 μg L and 0.09 μg L for Cr(III) and Cr(VI), respectively, at 52 under He collision mode.
Authors
Keywords
No keywords available for this article.