Machine learning-enhanced assessment of potential probiotics from healthy calves for the treatment of neonatal calf diarrhea.

Journal: Frontiers in microbiology
Published Date:

Abstract

Neonatal calf diarrhea (NCD) remains a significant contributor to calf mortality within the first 3 weeks of life, prompting widespread antibiotic use with associated concerns about antimicrobial resistance and disruption of the calf gut microbiota. Recent research exploring NCD treatments targeting gut microbiota dysbiosis has highlighted probiotic supplementation as a promising and safe strategy for gut homeostasis. However, varying treatment outcomes across studies suggest the need for efficient treatment options. In this study, we evaluated the potential of probiotics , formally known as , isolated from healthy neonatal calves to treat NCD. Through whole genome analysis and assays, we identified nine strains, which were then administered to calves with NCD. Calves treated with strains shed healthy feces and demonstrated restored gut microbiota and normal animal behavior. Leveraging a machine learning model, we evaluated microbiota profiles and identified bacterial taxa associated with calf gut health that were elevated by administration. These findings represent a crucial advancement towards sustainable antibiotic alternatives for managing NCD, contributing significantly to global efforts in mitigating antimicrobial resistance and promoting overall animal health and welfare.

Authors

  • Yuting Zhai
    Department of Animal Sciences, University of Florida, Gainesville, FL, United States.
  • Miju Kim
    Department of Animal Sciences, University of Florida, Gainesville, FL, United States.
  • Peixin Fan
    Department of Animal Sciences, University of Florida, Gainesville, FL, United States.
  • Sharath Rajeev
    Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States.
  • Sun Ae Kim
    Department of Food Science and Biotechnology, Ewha Womans University, Seoul, Republic of Korea.
  • J Danny Driver
    Department of Animal Sciences, University of Florida, Gainesville, FL, United States.
  • Klibs N Galvão
    Department of Large Animal Clinical Sciences, University of Florida, Gainesville, FL, United States.
  • Christina Boucher
    Department of Computer and Information Science and Engineering, University of Florida, Gainesville, FL, United States.
  • Kwangcheol C Jeong
    Department of Animal Sciences, University of Florida, Gainesville, FL, United States.

Keywords

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