Application of Single-cell Deep Learning in Elucidating the Mapping Relationship Between Visceral and Body Surface Inflammatory Patterns
Journal:
arXiv
Published Date:
Mar 21, 2025
Abstract
As a system of integrated homeostasis, life is susceptible to disruptions by
visceral inflammation, which can disturb internal environment equilibrium. The
role of body-spread subcutaneous fascia (scFascia) in this process is poorly
understood. In the rat model of Salmonella-induced dysentery, scRNA-seq of
scFascia and deep-learning analysis revealed Warburg-like metabolic
reprogramming in macrophages (MPs) with reduced citrate cycle activity.
Cd34+/Pdgfra+ telocytes (CPTCs) regulated MPs differentiation and proliferation
via Wnt/Fgf signal, suggesting a pathological crosstalk pattern in the
scFascia, herein termed the fascia-visceral inflammatory crosstalk pattern
(FVICP). PySCENIC analysis indicated increased activity transcription factors
Fosl1, Nfkb2, and Atf4, modulated by CPTCs signaling to MPs, downregulating
aerobic respiration and upregulating cell cycle, DNA replication, and
transcription. This study highlights scFascia's role in immunomodulation and
metabolic reprogramming during visceral inflammation, underscoring its function
in systemic homeostasis.
