Biocompatibility of colloidal polypyrrole.

Journal: Colloids and surfaces. B, Biointerfaces
Published Date:

Abstract

The excellent electroconductive properties of polypyrrole (PPy) predetermine its use as cell-instructive or biosensing biomaterial. PPy has potential for application in a physiological environment, and here the cytotoxicity, antioxidant capacity, modulation of neutrophil oxidative burst, antibacterial activity of colloidal PPy stabilized with polyvinylpyrrolidone (PVP), and long-term stability under physiological conditions are reported. Besides, long-term stability under physiological conditions is also provided. The formation of PPy/PVP colloid was proven by UV-Vis and IR spectroscopy, and its morphology and particle size were detected. The results of cytotoxicity studies show that the safe PPy concentration in colloidal particles for biomedical usage is ≤ 200 µg mL. In these concentrations, the PPy/PVP also exhibits excellent antioxidant properties and modulates the immune response. The minimal inhibitory concentration of 234 µg mL was determined against Staphylococcus aureus and Escherichia coli, respectively. The low cytotoxicity and bioactivity of the colloidal PPy/PVP demonstrate their long-term potential for a variety of applications.

Authors

  • Simona Káčerová
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic.
  • Zdenka Víchová
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic. Electronic address: vichova@utb.cz.
  • Kristýna Valášková
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic.
  • Jan Vícha
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic.
  • Lukáš Münster
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic.
  • Věra Kašpárková
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic; Faculty of Technology, Tomas Bata University in Zlin, Vavrečkova 5669, 76001 Zlin, Czech Republic.
  • Ondřej Vašíček
    Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 61200 Brno, Czech Republic. Electronic address: ondrej.vasicek@ibp.cz.
  • Petr Humpolíček
    Centre of Polymer Systems, Tomas Bata University in Zlin, nám. T. G. Masaryka 5678, 76001 Zlin, Czech Republic; Faculty of Technology, Tomas Bata University in Zlin, Vavrečkova 5669, 76001 Zlin, Czech Republic.

Keywords

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