Normal Patch Retinex Robust Alghoritm for White Balancing in Digital Microscopy
Journal:
arXiv
Published Date:
Jul 8, 2025
Abstract
The acquisition of accurately coloured, balanced images in an optical
microscope can be a challenge even for experienced microscope operators. This
article presents an entirely automatic mechanism for balancing the white level
that allows the correction of the microscopic colour images adequately. The
results of the algorithm have been confirmed experimentally on a set of two
hundred microscopic images. The images contained scans of three microscopic
specimens commonly used in pathomorphology. Also, the results achieved were
compared with other commonly used white balance algorithms in digital
photography. The algorithm applied in this work is more effective than the
classical algorithms used in colour photography for microscopic images stained
with hematoxylin-phloxine-saffron and for immunohistochemical staining images.