Membrane-Penetrating Molecular Device Based on a Triplex Containing Acyclic L-Threoninol Nucleic Acid Functionalized with Cholesterol.
Journal:
Angewandte Chemie (International ed. in English)
Published Date:
Nov 27, 2025
Abstract
Membrane-penetrating molecular devices are valuable biological tools. Herein, we describe membrane-targeting molecular devices based on the triplexes of poly T acyclic L-threoninol nucleic acid (L-aTNA) tethered site-specifically to cholesterol and poly A DNA. The L-aTNA was linked to cholesterol in two different orientations. The membrane-binding properties differed depending on the orientation at the cholesterol linkage. One triplex adhered to giant unilamellar vesicles and the other penetrated these vesicles. When the membrane-penetrating triplex was formed with a photoresponsive DNA, photoresponsive signal transduction into giant unilamellar vesicles was enabled. These membrane-targeting molecular devices have potential as biosensors, artificial organs, and molecular robots and in chemical artificial intelligence. Moreover, this strategy for post-modification of the propargyl-L-aTNA unit will enable further functionalization of L-aTNA and other acyclic XNAs.
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