Longitudinal immune transcriptomic signatures are associated with carotid intima-media thickness over 18 years
Journal:
medRxiv
Published Date:
Mar 13, 2026
Abstract
Background. Atherosclerosis is increasingly recognized as a chronic immunometabolic disorder involving complex interactions between circulating immune cells, metabolic factors, and the vascular wall. Carotid intima-media thickness (IMT) is widely used as a surrogate marker of subclinical atherosclerosis. Peripheral blood mononuclear cells (PBMCs) provide a systemic readout of immune transcriptional states, but longitudinal evidence linking PBMC transcriptional profiles to long-term vascular remodeling remains limited. Methods. We analyzed the association between PBMC transcriptomic profiles and carotid IMT in the Barilla Offspring Study, a single-center cohort with long-term follow-up. PBMC transcriptomics and carotid IMT were assessed at baseline in 148 participants, and 101 individuals underwent repeat clinical, vascular, and transcriptomic evaluation at follow-up. Three analytical configurations were examined: baseline cross-sectional, follow-up cross-sectional, and a longitudinal model linking baseline transcriptomic profiles to follow-up IMT. Following a comparison of state-of-the-art machine learning regression algorithms and an innovative rank-based method, the most predictive transcriptomic signature from each analytical configuration was used for downstream functional enrichment and network analyses. Results. The rank-based regression method showed the best performance across all analytical configurations. Cross-sectional analyses at both time points consistently revealed enrichment of immune-related pathways, including leukocyte activation, antigen presentation, and receptor-mediated signaling. In contrast, the longitudinal transcriptomic signature was enriched for pathways related to metabolic regulation, redox processes, and cellular structural organization. Despite limited overlap at the single-gene level, functional similarity analysis demonstrated convergence toward shared immunometabolic pathways associated with vascular remodeling. Conclusions. PBMC transcriptional profiles are associated with subclinical vascular remodeling both cross-sectionally and over long-term follow-up, suggesting that systemic immune transcriptional states may contribute to vascular aging.
