Next-generation discovery: empowering organoid research with machine learning, artificial intelligence, and mathematical modeling.

Organoids have rapidly matured into powerful model systems. The field is pushing organoids toward architectural sophistication and functional fidelity, with longitudinal experiments producing ever-larger and more complex datasets. As a result, computational methods have become indispensable for experimental design, data analysis, and predictive modeling, as well as for obtaining mechanistic insights. In this review, we survey recent progress at the interface of organoid research and computational approaches, discuss key challenges on both fronts, and outline future directions to maximize impact in biomedical research through convergent, synergistic efforts.