Physicochemical differences between camelid single-domain antibodies and mammalian antibodies.

Journal: Turkish journal of biology = Turk biyoloji dergisi
Published Date: Dec 7, 2023

Abstract

BACKGROUND/AIM: In recent years, single-domain antibodies, also known as nanobodies, have emerged as an alternative to full immunoglobulin Gs (IgGs), due to their various advantages, including increased solubility, faster clearance, and cheaper production. Nanobodies are generally derived from the variable domain of the camelid heavy-chain-only immunoglobulin Gs (hcIgGs). Due to the high sequence homology between variable heavy chains of camelids (VHs) and humans (Vs), hcIgGs are ideal candidates for nanobody development. However, further examination is needed to understand the structural differences between Vs and VHs. This analysis is essential for nanobody engineering to mitigate potential immunogenicity, while preserving stability, functionality, and antigen specificity.

Authors

  • Nazlı Eda Eskier
    İzmir International Biomedicine and Genome Institute, Dokuz Eylül University, İzmir, Turkiye.
  • Doğa Eskier
    İzmir International Biomedicine and Genome Institute, Dokuz Eylül University, İzmir, Turkiye.
  • Esin Firuzan
    Department of Statistics, Faculty of Sciences, Dokuz Eylül University, İzmir, Turkiye.
  • Sibel Kalyoncu Uzunlar
    İzmir Biomedicine and Genome Center, İzmir, Turkiye.

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