SUMA: a lightweight machine learning model-powered shared nearest neighbour-based clustering application interface for scRNA-Seq data.
Journal:
Turkish journal of biology = Turk biyoloji dergisi
Published Date:
Dec 18, 2023
Abstract
BACKGROUND/AIM: Single-cell transcriptomics (scRNA-Seq) explores cellular diversity at the gene expression level. Due to the inherent sparsity and noise in scRNA-Seq data and the uncertainty on the types of sequenced cells, effective clustering and cell type annotation are essential. The graph-based clustering of scRNA-Seq data is a simple yet powerful approach that presents data as a "shared nearest neighbour" graph and clusters the cells using graph clustering algorithms. These algorithms are dependent on several user-defined parameters.Here we present SUMA, a lightweight tool that uses a random forest model to predict the optimum number of neighbours to obtain the optimum clustering results. Moreover, we integrated our method with other commonly used methods in an RShiny application. SUMA can be used in a local environment (https://github.com/hkarakurt8742/SUMA) or as a browser tool (https://hkarakurt.shinyapps.io/suma/).
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