Characterization of a -targeting lytic bacteriophage SSJ01 and its application in artificial seawater.
Journal:
Food science and biotechnology
Published Date:
Nov 20, 2023
Abstract
is a major foodborne pathogen causing serious illnesses. In this study, a new lytic bacteriophage SSJ01 that infects was isolated and characterized. It had a short non-contractile tail and belonged to the class. It rapidly adsorbed onto host cells, exhibited a short latent period, and has a large burst size. It showed lytic activities under a broad range of temperature (- 18 to 60 °C), pH (5 to 11), and salinity (0 to 6%). It contained 35 open reading frames with a G + C content of 49.16% without toxic or lysogen-forming genes. The MOI of 10 phage-treated group in vitro reduced the target cells up to 3.49-log CFU/mL at 6 °C and 3.47-log CFU/mL at 25 °C, respectively. In aquatic environments (6 and 25 °C), bactericidal activities showed a significant decrease within 2 h. Therefore, the bacteriophage SSJ01 has potential as a biocontrol agent to control in marine culture.
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