Membrane proteomics of the Drosophila circadian neural network
Journal:
bioRxiv
Published Date:
May 21, 2026
Abstract
Circadian behaviors are controlled by dedicated brain pacemaker neurons, whose activity oscillate during the day and the night. The Drosophila brain contains ca. 240 such neurons. Their molecular clock is synchronized, but the phase of their rhythmic neural activity differs dramatically between functional groups. This explains how specific circadian neurons can for example promote morning or evening locomotor activity. To understand in depth how the circadian network functions, we surveyed its membrane proteome in the morning and evening, using in situ protein labeling and mass spectrometry. In addition to detecting known regulators of circadian behavior, we identified novel membrane or membrane-associated proteins present in circadian neurons. Through genetic screens, we found that many of these proteins regulate circadian behavior. In particular, Piezo regulates morning activity specifically under short photoperiod, and its loss compromises the structural plasticity of the clock neurons controlling locomotion at dawn. Our work thus illustrates the power of proteome-guided genetic screens to understand the mechanisms underlying circadian behavior.
