Development of a high-resolution melt-based assay to rapidly detect the azole-resistant isolates.

Journal: Current medical mycology
Published Date: Sep 1, 2023

Abstract

BACKGROUND AND PURPOSE: is a multidrug-resistant yeast that rapidly spreads, making it the leading Candidate for the next pandemic. One main leading cause of emerging resistant isolates is nonsynonymous mutations. This study aimed to detect the Y132F mutation, one of the most important azole resistance-associated mutations in the gene of , by developing a reliable high-resolution melt (HRM)-based method.

Authors

  • Hamid Morovati
    Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Hamid Badali
    Department of Molecular Microbiology and Immunology, South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, San Antonio, TX, USA.
  • Mahdi Abastabar
    Invasive Fungi Research Center, Communicable Diseases Research Institute, Mazandaran University of Medical Sciences, Sari, Iran.
  • Keyvan Pakshir
    Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Kamiar Zomorodian
    Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran.
  • Bahram Ahmadi
    Department of Medical Laboratory Sciences, Faculty of Paramedicine, Bushehr University of Medical Sciences, Bushehr, Iran.
  • Behrouz Naeimi
    Department of Medical Laboratory Sciences, Faculty of Paramedicine, Bushehr University of Medical Sciences, Bushehr, Iran.
  • Sadegh Khodavaisy
    Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Sanam Nami
    Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Esmaeil Eghtedarnejad
    Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Hossein Khodadadi
    Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Keywords

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