Simultaneous detection of human norovirus GI, GII and SARS-CoV-2 by a quantitative one-step triplex RT-qPCR.

Journal: Frontiers in microbiology
Published Date: Jan 8, 2024

Abstract

BACKGROUND: There are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-CoV-2, a quantitative one-step triplex reverse transcription PCR (RT-qPCR) method was designed in this paper.

Authors

  • Hua-Wei Yi
    The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
  • Xian-Mo Wang
    The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
  • Xin Tan
    School of Public Health, Chengdu Medical College, Chengdu 610500, China.
  • Cai-Zhi Ding
    The People's Hospital of Songzi, Jingzhou, Hubei, China.
  • Chang-Li Zhang
    The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
  • Jia-Hao Wu
    The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
  • Qi Li
    The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
  • Chen-Qi Xin
    The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei, China.
  • Wen Fan
    Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Keywords

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