Enhanced exopolysaccharide yield and antioxidant activities of fermented products by the addition of Radix Puerariae.

Journal: RSC advances
Published Date: Nov 26, 2021

Abstract

To increase the production of exopolysaccharides (EPS) and expand the application of () fermentation liquid, the traditional Chinese medicine Radix Puerariae (RP) with outstanding biological activity was selected as a culture additive to improve the EPS yield and enhance the antioxidant activity of fermented products from . The effects of three independent factors: A: initial pH (5.0-6.0), B: concentration of RP (10-14 g L), and C: inoculum size (8-12%, v/v) on the EPS yield were evaluated. The results of response surface methodology (RSM) showed that the optimal fermentation conditions were: A: 5.40, B: 12.80 g L, and C: 10.0%. The optimal yield of EPS was 8.41 ± 0.12 mg mL, which showed an insignificant ( > 0.05) difference with the predicted value (8.45 mg mL). The fermented supernatants cultured from RP-supplemented medium (SC-RP) or regular medium (SC) were collected for further study. FT-IR analysis of EPS-1 (purified from SC) and EPS-2 (purified from SC-RP) showed that their structures were consistent, indicating that the addition of RP did not affect the structure of schizophyllan (SPG). In addition, compared with SC, the antioxidant activities of SC-RP were significantly improved with ORAC values and FRAP values increasing by 11.56-fold and 14.69-fold, respectively. There was a significant correlation among the phenolic compounds, flavonoids, and antioxidant activity of SC-RP in this study. Besides, SC-RP was detected to contain more than 25 bioactive ingredients compared with that of SC, which may play a key role in its antioxidant activities. Thus, these results indicated that RP enhanced the yield of SPG and improved the antioxidant activity of the fermented products by . Accordingly, the fermentation liquid of with the addition of RP may have potential application in food, cosmetics, and pharmaceutical industries.

Authors

  • Yongfei Deng
    School of Light Industry and Food Engineering, Guangxi University Nanning 530004 People's Republic of China dj6688@126.com.
  • Qian Huang
    Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
  • Lu Hu
    Research and Development Center, Guangdong Marubi Biotechnology Co., Ltd Guangzhou 510700 People's Republic of China guo.chaowan@marubi.cn.
  • Tao Liu
    Institute of Urology and Nephrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
  • Bisheng Zheng
    School of Food Science and Engineering, South China University of Technology Guangzhou 510641 People's Republic of China.
  • Dengjun Lu
    School of Light Industry and Food Engineering, Guangxi University Nanning 530004 People's Republic of China dj6688@126.com.
  • Chaowan Guo
    Research and Development Center, Guangdong Marubi Biotechnology Co., Ltd Guangzhou 510700 People's Republic of China guo.chaowan@marubi.cn.
  • Lin Zhou
    Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University Guangzhou 510006 People's Republic of China zhoulin@gdpu.edu.cn +86-20-39352151 +86-20-39352151.

Keywords

No keywords available for this article.

External Resources

Popular Topics
Recent Journals