Modification of a nitrocellulose membrane with nanofibers for sensitivity enhancement in lateral flow test strips.

Journal: RSC advances
Published Date: Aug 2, 2021

Abstract

Lateral-flow analysis (LFA) is a convenient, low-cost, and rapid detection method, which has been widely used for screening of diseases. However, sensitivity enhancement in LFA is still a focus in this field and remains challenging. Herein, we propose an electrospinning coating method to modify the conventional nitrocellulose (NC) membrane and optimize the liquid flow rate for enhancing the sensitivity of the NC based LFA strips in the detection of human chorionic gonadotropin (HCG) and luteinizing hormone (LH). It can be seen that coating the NC membrane with nitrocellulose fibers could obtain a NC based strip with HCG and LH detection limits of 0.22 and 0.36 mIU mL respectively, and a quantitative linear range of 0.5-500 mIU mL. The results show that electrospinning is effective in modifying conventional NC membranes for LFA applications.

Authors

  • Xue Wang
    Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening Biology Institute, Qilu University of Technology (Shandong Academy of Sciences) Jinan China.
  • Chao-Hua Xue
    College of Environmental Science and Engineering, Shaanxi University of Science and Technology Xi'an 710021 China xuechaohua@126.com.
  • Dong Yang
    College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology Xi'an 710021 China yangdong@sust.edu.cn.
  • Shun-Tian Jia
    College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology Xi'an 710021 China.
  • Ya-Ru Ding
    College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology Xi'an 710021 China.
  • Lei Lei
    Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • Ke-Yi Gao
    College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology Xi'an 710021 China yangdong@sust.edu.cn.
  • Tong-Tong Jia
    College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology Xi'an 710021 China yangdong@sust.edu.cn.

Keywords

No keywords available for this article.

External Resources

Popular Topics
Recent Journals