Isolation and Identification of Polyphenols From Fresh Sweet Sorghum Stems and Their Antibacterial Mechanism Against Foodborne Pathogens.

Journal: Frontiers in bioengineering and biotechnology
Published Date: Feb 11, 2022

Abstract

As a C4 energy crop widely planted all over the world, sweet sorghum is mainly used in sugar making and brewing. Fresh sweet sorghum stalks contain many natural ingredients that have antioxidant properties and can significantly inhibit the growth of foodborne pathogens. In this study, the polyphenols in sweet sorghum were extracted by acid ethanol and ion precipitation, and the types of polyphenols were determined by HPLC-MS. The polyphenol content in fresh sweet sorghum stalks was 5.77 mg/g after process optimization with 18 types of phenolic acids identified. The extract had a total antioxidant capacity of 9.4 μmol Trolox/mL. Polyphenol extract of sweet sorghum displayed antibacterial activity against spp. and spp. The extract increased the conductivity of cell suspensions by destroying the membrane structure, resulting in leakage of cell electrolytes. Changes in bacterial morphology and internal structure were indicated. The data describe an optimized process to extract polyphenols from sweet sorghum stalks and the methodology to identify the major components within the extract. The data provide a novel option for the comprehensive utilization of fresh sweet sorghum stalks.

Authors

  • Hao Chen
    The First School of Medicine, Wenzhou Medical University, Wenzhou, China.
  • Yifei Xu
    Gansu Institute of Food Inspection, Lanzhou, China.
  • Haoyu Chen
    Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
  • Hao Liu
    Key Laboratory of Development and Maternal and Child Diseases of Sichuan Province, Department of Pediatrics, Sichuan University, Chengdu, China.
  • Qunli Yu
    College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
  • Ling Han
    School of Land Engineering, Chang'an University, Xi'an 710064, China; Xi'an Key Laboratory of Territorial Spatial Information, School of Land Engineering, Chang'an University, Xi'an 710064, China. Electronic address: hanling@chd.edu.cn.

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