Phytosynthesis of silver nanoparticles from Jacq. flower extract and their possible applications as antibacterial and antioxidant agent.

Journal: Saudi journal of biological sciences
Published Date:

Abstract

 Jacq. flower extract was used for the synthesis of silver nanoparticles in the current study. Various spectroscopic analyses were used to characterize the synthesized nanoparticles (JIF-AgNPs). The antibacterial efficacy of JIF-AgNPs was studied by well diffusion and microdilution techniques. In addition, the impact of JIF-AgNPs on free radicals was evaluated. On the ultraviolet-visible spectrum, the nanoparticles exhibit the highest absorbance at 422 nm. Based on the Fourier transform infrared spectrum, phenols and amino acids were involved in capping the JIF-AgNPs. Crystalline sphere-shaped nanoparticles with an average size of 50.07 nm and zeta potential of -19.0 mV were confirmed by X-ray diffraction, transmission electron microscopy, and dynamic light scattering analysis respectively. The JIF-AgNPs exhibit the highest and lowest growth inhibitory activity towards  and . The minimal inhibitory concentration of JIF-AgNPs against , and  were 2.5, 5.0, 5.0, and 7.5 μg/mL, respectively. The JIF-AgNPs exhibited significant radical scavenging activities against DPPH (IC-32.5 ± 0.06 µg/mL), hydroxyl (IC-25 ± 0.09 µg/mL), Superoxide (IC-42.5 ± 0.13 µg/mL), and ABTs (IC-33.5 ± 0.15 µg/mL). Thus, synthesized nanoparticles were a good alternative to develop an antibacterial and antioxidant agent.

Authors

  • Gunasekaran Suriyakala
    Nano and Energy Bioscience Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India.
  • Sivaji Sathiyaraj
    Nano and Energy Bioscience Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India.
  • Sandhanasamy Devanesan
    Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box - 2455, Riyadh 11451, Saudi Arabia.
  • Mohamad S AlSalhi
    Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box - 2455, Riyadh 11451, Saudi Arabia.
  • Aruliah Rajasekar
    Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore, Tamilnadu 632115, India.
  • Murali Kannan Maruthamuthu
    Department of Biotechnology & Bioengineering, Purdue University, 610 Purdue Mall, West Lafayette, IN 47907, United States.
  • Ranganathan Babujanarthanam
    Nano and Energy Bioscience Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamil Nadu, India.

Keywords

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