Clinical Assessment and Validation of a Rapid and Sensitive SARS-CoV-2 Test Using Reverse Transcription Loop-Mediated Isothermal Amplification Without the Need for RNA Extraction.

Journal: Open forum infectious diseases
Published Date: Dec 21, 2020

Abstract

BACKGROUND: Amid the enduring pandemic, there is an urgent need for expanded access to rapid, sensitive, and inexpensive coronavirus disease 2019 (COVID-19) testing worldwide without specialized equipment. We developed a simple test that uses colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute resrpiratory syndrome coronavirus 2 (SARS-CoV-2) in 40 minutes from sample collection to result.

Authors

  • Melis N Anahtar
    Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
  • Graham E G McGrath
    Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Brian A Rabe
    Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts, USA.
  • Nathan A Tanner
    New England Biolabs, Ipswich, Massachusetts, USA.
  • Benjamin A White
    Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Jochen K M Lennerz
    Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
  • John A Branda
    Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
  • Constance L Cepko
    Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts, USA.
  • Eric S Rosenberg
    Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

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