Development and Validation of a Novel LC-MS/MS Assay for C-Peptide in Human Serum.

Journal: Journal of mass spectrometry and advances in the clinical lab
Published Date: Dec 28, 2020

Abstract

INTRODUCTION: C-peptide is used as a marker of endogenous insulin secretion in the assessment of residual β-cell function in diabetes and in the diagnostic workup of hypoglycemia. Previously developed LC-MS/MS methods to quantify serum concentrations of C-peptide have monitored intact peptide, which ionizes poorly. As a result, methods have leveraged immunoaffinity enrichment or two-dimensional chromatography. In this study, we aimed to use proteolysis during sample preparation to enhance the sensitivity of traditional LC-MS/MS.

Authors

  • Benjamin Y Owusu
    Department of Laboratory Medicine, University of Washington, Seattle, WA.
  • Hannah Pflaum
    Department of Laboratory Medicine, University of Washington, Seattle, WA.
  • Russell Garner
    Department of Laboratory Medicine, University of Washington, Seattle, WA.
  • North Foulon
    Department of Laboratory Medicine, University of Washington, Seattle, WA.
  • Thomas J Laha
    Department of Laboratory Medicine, University of Washington, Seattle, WA.
  • Andrew N Hoofnagle
    Department of Laboratory Medicine, University of Washington, Seattle, WA.

Keywords

No keywords available for this article.

External Resources

Popular Topics
Recent Journals