Hydroxyl Radical Generation Through the Fenton-Like Reaction of Hematin- and Catechol-Functionalized Microgels.

Journal: Chemistry of materials : a publication of the American Chemical Society
Published Date: Sep 4, 2020

Abstract

Hydroxyl radical (OH) is a potent reactive oxygen species with the ability to degrade hazardous organic compounds, kill bacteria, and inactivate viruses. However, an off-the-shelf, portable, and easily activated biomaterial for generating OH does not exist. Here, microgels were functionalized with catechol, an adhesive moiety found in mussel adhesive proteins, and hematin (HEM), a hydroxylated Fe ion-containing porphyrin derivative. When the microgel was hydrated in an aqueous solution with physiological pH, molecular oxygen in the solution oxidized catechol to generate HO, which was further converted to OH by HEM. The generated OH was able to degrade organic dyes, including orange II and malachite green. Additionally, the generated OH was antimicrobial against both gram-negative () and gram-positive () bacteria with the initial concentration of 10-10 CFU/mL. These microgels also reduced the infectivity of a non-enveloped porcine parvovirus and an enveloped bovine viral diarrhea virus by 3.5 and 4.5 log reduction values, respectively (99.97-99.997% reduction in infectivity). These microgels were also functionalized with positively charged [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC), which significantly enhanced the antibacterial and antiviral activities through electrostatic interaction between the negatively charged pathogens and the microgel. These microgels can potentially serve as a lightweight and portable source of disinfectant, for an on-demand generation of OH with a wide range of applications.

Authors

  • Pegah Kord Forooshani
    Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Rattapol Pinnaratip
    Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Elizabeth Polega
    Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Ariana G Tyo
    Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Eric Pearson
    Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Bo Liu
    Wuhan United Imaging Healthcare Surgical Technology Co., Ltd., Wuhan, China.
  • Tinu-Ololade Folayan
    Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Lei Pan
    Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Rupak M Rajachar
    Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Caryn L Heldt
    Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, USA.
  • Bruce P Lee
    Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931, USA.

Keywords

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