Mediated Synthesis of Silver Nanoparticles and Its Evaluation of Antineoplastic Activity to Combat Colorectal Cancer Cell Line.
Journal:
Iranian journal of pharmaceutical research : IJPR
Published Date:
Jan 1, 2020
Abstract
Several formulations of herbal plants have been extensively applied to treat diseases. ( is an Iranian traditional plant with a wide range of benefit effects on different diseases. In this study, we aimed to prepare silver nanoparticles from via the green synthesis method and investigate the anti-cancer effects on the HT29 cell line. To synthesize Ag- 50 mL extract and 1 mM AgNO3 were mixed and shaken at room temperature for 72 h. To determine the Ag- nanoparticle characterization, XRD, FTIR, and TEM methods were done. In addition, MTT assay and real time PCR and annexin V/PI staining were performed to investigate the cytotoxicity, and gene expression and percentage of apoptotic cells. Our findings showed that Ag- is a spherical crystalline nanoparticle with the size less than 100 nm. MTT analysis showed that 375, 750, 1500 and 3000 µg/mL significantly decreased the cell viability of HT29 cells. Ag- significantly reduced and increased apoptotic index expression at 375, 750, 1500, 3000 µg/mL in a dose-dependent manner. Furthermore, cell staining with Annexin V/PI showed that treating Ag led to increasing in apoptotic cells. In conclusion, the formulation of Ag- has the apoptotic properties on the colorectal cancer cell line.
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