Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of .

Journal: Current medical mycology
Published Date: Sep 1, 2020

Abstract

BACKGROUND AND PURPOSE: is an emerging multidrug-resistant pathogen. The identification of this species with the conventional phenotypic or biochemical mycological methods may lead to misidentification. Molecular-based species-specific identification methods such as quantitative real-time polymerase chain reaction (qPCR) facilitate a more reliable identification of than mycological methods. Regarding this, the present study aimed to develop a hydrolysis probe-based qPCR assay for the rapid, accurate identification of .

Authors

  • Hadis Jafarian
    Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Hossein Khodadadi
    Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Parisa Badiee
    Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

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