A Low-Cost Nanomaterial-based Electrochemical Immunosensor on Paper for High-Sensitivity Early Detection of Pancreatic Cancer.

Journal: Sensors and actuators. B, Chemical
Published Date: Dec 2, 2019

Abstract

Due to the lack of specific early detection methods for pancreatic cancer, it usually goes undetected until it is advanced. By employing paper-based electrodes (PPE), herein we for the first time developed a disposable low-cost paper-based immunosensor for rapid early quantitative detection of pancreatic cancer with a new biomarker, pseudopodium-enriched atypical kinase one, SGK269 (PEAK1). The immunosensor was constructed by fabricating PPEs immobilized with the versatile nanomaterial graphene oxide for the incorporation of antibodies to form an immunosensing platform, without the need of complicated surface modification. After it was confirmed that the PPEs exhibited excellent electrochemical properties, a sandwich-type electrochemical immunosensor was subsequently constructed by employing graphene oxide layers immobilized with anti-PEAK1, and the antibody conjugated with gold nanoparticles (AuNPs-tagged-Anti PEAK1). Further, spectral and surface characteristic studies confirmed the formation of the immunosensing platform. The immunosensor for PEAK1 exhibited a wide linear range between 10 pg mL and 10 pg mL with a low limit of detection (LOD) of 10 pg mL. The obtained results point towards rapid, sensitive, and specific early diagnosis of pancreatic cancer at the point of care and other low-resource settings.

Authors

  • K Sudhakara Prasad
    Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.
  • Xiyue Cao
    Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.
  • Ning Gao
    Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.
  • Qijie Jin
    Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.
  • Sharma T Sanjay
    Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.
  • Gilberto Henao-Pabon
    Biomedical Engineering, Border Biomedical Research Center, and Environmental Science & Engineering, University of Texas at El Paso, USA.
  • XiuJun Li
    Department of Chemistry & Biochemistry, University of Texas at El Paso, Texas, USA.

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