Antimicrobial Activities of Extracts and Isolated Coumarins from the Roots of Four Species Growing in Turkey.
Journal:
Iranian journal of pharmaceutical research : IJPR
Published Date:
Jan 1, 2019
Abstract
species have been utilized since ancient times as digestive, sedative, aphrodisiac, along with in salads or as a spice due to their special odors. The study reports isolation and characterization of bioactive compounds of , (, and ( via bioassay guided fractionation and isolation process. The structures of compounds were elucidated by detailed spectroscopic analyses. They were also assessed for their activities at 1000-31.25 µg/mL concentrations by microbroth-dilution methods. Antimicrobial activity of aqueous, methanol extracts and dichloromethane, ethyl acetate, -butanol and aqueous residue fractions of methanol extracts from aerial parts and roots of species along with isolated compounds [osthole, imperatorin, bergapten, prantschimgin, peucedanol-2'-benzoate, grandivitinol, suberosin, xanthotoxin, felamidin, marmesin, umbelliferone, ulopterol and a sterol mixture consisted of stigmasterol, -sitosterol] were evaluated. Antimicrobial effect has been seen against Gram-negative, Gram-positive bacteria, and a yeast at a concentration between 31.25 and 62.5 μg/mL. Especially, (MIC = 31.25 μg/mL) was the most inhibited microorganism. Moreover, growth of , , and were inhibited at 62.5 μg/mL MIC values. Among tested samples prantschimgin and dichloromethane fraction of aerial parts from showed the best activity against (MIC = 31.25 μg/mL). However, among aqueous extracts and residue fractions, only aerial parts, aerial parts, and roots and roots showed activity against . Among microorganisms was found to be the least affected.
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