Intrinsic Conformation-Induced Fluorescence Resonance Energy Transfer Aptasensor.

Journal: ACS applied bio materials
Published Date: Oct 3, 2019

Abstract

The emergence of aptamer probes would potentially evolve the tools for the analysis of biomarkers, environmental and food contaminations, however, their application still suffers from the complex design of nucleic acid structures. Herein, we propose an intrinsic conformation-induced aptasensor (iAptasensor). The single aptamer sequence-featured aptasensor could perform with the direct use of aptamer sequence, thus eliminating the trial-and-error test of aptamer probe design. Remarkably, it endows with both conformation and distance effects response to target molecules via fluorescence resonance energy transfer (FRET) mechanism, synergistically amplifying the detection signals, in turn enhancing the sensitivity for aflatoxin B (AFB) detection (LOD of 0.32 ng/mL). The detection process could be finished with one-mixing step at room temperature, obviating the temperature-control and separation procedures. Furthermore, iAptasensor allowed researchers to analyze AFB in different environmental and food samples with recovery rates of 89.2% to 110.8%, indicating the reliability and feasibility of iAptasensor for detecting AFB contaminations in complex samples.

Authors

  • Hao Yang
    College of Agricultural Science and Engineering, Hohai University, Nanjing 210098, China.
  • Wenyue Zhao
    College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
  • Sha Deng
    College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
  • Kaixiang Zhang
    Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.
  • Zhifeng Zhao
    College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
  • Ruijie Deng
    College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
  • Qiang He
    College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
  • Jinghong Li
    Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.

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