Characterization and antifungal activity of the yellow pigment produced by a sp. DBS4 isolated from the lichen .

Journal: Saudi journal of biological sciences
Published Date: Nov 29, 2019

Abstract

This study emphasis the production of yellow pigment from endolichenic sp. isolated from the lichen (Afzel. ex Ach.) B.J. Moore Yellow pigment-producing twenty different strains were investigated. The hyperactive pigment-producing bacterial strain was identified as based on 99 % sequence similarity. Maximum bacterial pigment production appeared in Luria Bertani medium. Methanol extraction of the pigment and its partial purification using TLC was carried out. Furthermore, isolated pigments were characterized using UV-visible spectroscopy, FTIR spectroscopy, and GC-MS results related to the possibility of the carotenoid occurrence. The pigment also exhibited efficient antifungal activity against selected fungal pathogens of economic importance. Likewise, the pigment extract evaluated for the total antioxidant potential using Phosphomolybdenum and Ferric reducing antioxidant power assay and the results represented in Ascorbic Acid Equivalent (AAE)- 21.45 ± 1.212 mg/mL. The SC of the pigment extract found to be 75.125 ± 0.18 µg/ml determined by the ABTS assay.

Authors

  • Turki M Dawoud
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Naiyf S Alharbi
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Aswani M Theruvinthalakal
    Department of Biotechnology, Kamaraj College of Engineering and Technology, K.Vellakulam - 625 701, Madurai, Tamil Nadu, India.
  • Aswani Thekkangil
    Department of Biotechnology, Kamaraj College of Engineering and Technology, K.Vellakulam - 625 701, Madurai, Tamil Nadu, India.
  • Shine Kadaikunnan
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Jamal M Khaled
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Taghreed N Almanaa
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Karthikumar Sankar
    Department of Biotechnology, Kamaraj College of Engineering and Technology, K.Vellakulam - 625 701, Madurai, Tamil Nadu, India.
  • Ganesh Moorthy Innasimuthu
    Department of Biotechnology, Kamaraj College of Engineering and Technology, K.Vellakulam - 625 701, Madurai, Tamil Nadu, India.
  • Khaled F Alanzi
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Shyam Kumar Rajaram
    Department of Biotechnology, Kamaraj College of Engineering and Technology, K.Vellakulam - 625 701, Madurai, Tamil Nadu, India.

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