Action mechanism of anti-wrinkle effect of methanol extract in human dermal fibroblast and keratinocyte cell lines.
Journal:
Toxicological research
Published Date:
Dec 4, 2019
Abstract
is a deciduous broad-leaf bush and endemic species widely found in Korea. Recently, we reported that methanol extract (RYME) had excellent antioxidant activity and inhibition of collagenase and elastase activity in cell-free system. In this study, we investigated the ability of RYME to control the mRNA and protein expression levels of the known skin wrinkle-related factors in cultured human dermal fibroblast and keratinocyte cell lines. Treatment with 100 or 200 μg/mL RYME strongly blocked the UVB-induced downregulation of type 1 collagen mRNA expression ( < 0.001) and partially blocked the UVB-induced upregulation of MMP-3 mRNA expression in HaCaT human keratinocytes ( < 0.05 or < 0.001). Treatment with RYME at 100 μg/mL considerably decreased MMP-1 mRNA expression in UVB-exposed HaCaT cells ( < 0.01). In HaCaT cells, RYME exhibited the potential to improve UV light-induced skin wrinkles. Moreover, RYME selectively inhibited the UVB-induced ERK-1/2 protein phosphorylation in CCD-986sk human dermal fibroblasts at 80 and 160 μg/mL. UV-induced ERK-1/2 protein phosphorylation is one of the major mechanisms of the generation of UV-induced skin wrinkles. Therefore, it is likely that the anti-skin wrinkling effect of RYME could be attributable to selective inhibition of UV induced ERK-1/2 protein phosphorylation.
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