Isolation, Identification and Characteristics of From Diseased Crucian Carp ().

Journal: Frontiers in microbiology
Published Date: Nov 26, 2019

Abstract

species often cause disease in farmed fish. In the present study, dominant bacteria were isolated from diseased crucian carp (). Based on this, a bacterial isolate was tentatively named CFJY-623. This isolate was identified as based on analysis of its morphological, physiological, and biochemical features, as well as 16S rRNA and gene sequences. Six virulence genes related to pathogenicity including aerolysin, cytotonic enterotoxins, elastase, glycerophospholipid: cholesterol acyltransferase, lipase, and serine protease were identified in this isolate. The median lethal dosage (LD50) of the CFJY-623 isolate for crucian carp was determined as 1.31 × 10 CFU/mL. Artificial experimental infection showed that the CFJY-623 isolate caused considerable histological lesions in the fish, including tissue cell degeneration, necrosis, and inflammatory cell infiltrating. Drug sensitivity testing showed that the isolate was susceptible to aminoglycosides, carbapenemes, and nitrofurans. Exploring its growing features showed that this isolate exhibited a high level of environmental adaptability. These results provided a scientific basis for the identification of and treatment for fish infected by this pathogen.

Authors

  • Feng Chen
    Department of Integrated Care Management Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
  • Jingfeng Sun
    Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.
  • Zhuoran Han
    Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.
  • Xijun Yang
    Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.
  • Jian-An Xian
    Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
  • Aijun Lv
    Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.
  • Xiucai Hu
    Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.
  • Hongyue Shi
    Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.

Keywords

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