Amperometric genosensor for culture independent bacterial count.

Journal: Sensors and actuators. B, Chemical
Published Date: Aug 8, 2019

Abstract

Bacterial plate count for general assessment of water quality requires lengthy bacterial culturing. We report here a new DNA induced current genosensor for culture independent total bacteria determination. Since the amount of bacterial DNA is correlated to the number of bacteria, the genosensor measures the amount of bacterial DNA to determine bacterial count. The approach relies on bacteria lysis to release DNA which can react with molybdate to form redox molybdophosphate and measured electrochemically. Analysis of and demonstrated that the DNA generated current is highly correlated with the level of bacteria lysis which was confirmed by spectrometric measurement. Culture independent measurement of bacterial load suggests limit of detection is 21.9 CFU/mL, with linear range from 3×10 to 3×10 CFU/mL and correlation coefficient of 0.992. For analysis, the detection limit is 25.1 CFU/mL with the same linear range. The use of electrochemical microbial DNA quantitation for culture independent bacterial count is a new approach, the genosensor measurement is rapid (within 1 h) and has potential use for analysis of broad-spectrum bacteria for various applications.

Authors

  • Xingxing Jiang
    Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, China, 410083.
  • Shuping Liu
    Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, China, 410083.
  • Minghui Yang
    Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, China, 410083.
  • Avraham Rasooly
    National Cancer Institute, National Institutes of Health, Rockville, Maryland 20850, United States.

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