Cardiac Troponin I: Ultrasensitive Detection Using Faradaic Electrochemical Impedance.

Journal: ACS omega
Published Date: Dec 12, 2018

Abstract

An electrochemical biosensor for the detection of cardiac troponin I, cTnI, an important cardiac biomarker, is described. A combination of a novel monoclonal antibody, mAb20B3, and a novel Ir(III)-based metal complex was used for detection using faradaic electrochemical impedance spectroscopy. A limit of detection of 10 ag/mL was achieved, which is significantly lower than established assays. The ability to detect these ultralow concentrations enables rapid and early stage detection of cardiac events and opens up the possibility of developing a point-of-care device.

Authors

  • Elaine Spain
    National Centre for Sensor Research and School of Biotechnology, Dublin City University, Collins Avenue, D09 Y5N0, 9 Dublin, Ireland.
  • Serena Carrara
    Universitè de Strasbourg, CNRS, ISIS & icFRC, 8 Allée Gaspard Monge, 67083 Strasbourg, France.
  • Kellie Adamson
    National Centre for Sensor Research and School of Biotechnology, Dublin City University, Collins Avenue, D09 Y5N0, 9 Dublin, Ireland.
  • Hui Ma
    National Centre for Sensor Research and School of Biotechnology, Dublin City University, Collins Avenue, D09 Y5N0, 9 Dublin, Ireland.
  • Richard O'Kennedy
    National Centre for Sensor Research and School of Biotechnology, Dublin City University, Collins Avenue, D09 Y5N0, 9 Dublin, Ireland.
  • Luisa De Cola
    Universitè de Strasbourg, CNRS, ISIS & icFRC, 8 Allée Gaspard Monge, 67083 Strasbourg, France.
  • Robert J Forster
    National Centre for Sensor Research and School of Biotechnology, Dublin City University, Collins Avenue, D09 Y5N0, 9 Dublin, Ireland.

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