Leaf Extracts and Their Quercetin Protect HepG2 Cell Lines Against CCL Induced Cytotoxicity.

Journal: Indian journal of clinical biochemistry : IJCB
Published Date: Apr 11, 2018

Abstract

The present study was carried out to evaluate the in vitro cytoprotective effects of and their isolated quercetin fraction to reduce the CCl (carbon tetrachloride) induced toxicity in HepG2 cell lines (Hepatocellular carcinoma G2). Silymarin was used as a standard drug to compare the protective effects of plant extracts in infected cell lines. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay, cell viability assay, leakage parameters [Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH)], lipid peroxidation and reduced glutathione (GSH) levels were used to find out the protection of human derived HepG2 cells against CCl-induced damage. The levels of cytotoxicity, viability and GSH were reduced. While the activities of AST, ALT, LDH and lipid peroxidation was increased in CCl-treated groups. The treatment of and their isolated quercetin fractions (100, 200, 300 µg/mL) decreased the elevated levels of all these parameters. The results of the present study suggest that the ethanolic extract of leaf and their isolated quercetin fractions can able to reduce the CCl-induced cytotoxicity in HepG2 cell lines.

Authors

  • K Vijayakumar
    Department of Biochemistry, M.I.E.T. Arts and Science College, Tiruchirappalli, Tamil Nadu 620 007 India.
  • R L Rengarajan
    2Department of Animal Science, Centre for Pheromone Technology, Bharathidasan University, Tiruchirappalli, Tamil Nadu India.
  • R Radhakrishnan
    3Department of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.
  • Shilu Mathew
    4Centre of Excellence in Genomic Medicine Research, King Abdul Aziz University, P.O. Box 80216, Jeddah, 21589 Kingdom of Saudi Arabia.
  • Ishtiaq Qadri
    5King Fahd Medical Research Centre, King Abdul Aziz University, P.O. Box 802160, Jeddah, 21589 Kingdom of Saudi Arabia.
  • A Vijaya Anand
    6Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore, Tamil Nadu 641 046 India.

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