Evaluation of the Bactericidal and Fungicidal Activities of Poly([2-(methacryloyloxy)ethyl]trimethyl Ammonium Chloride)(Poly (METAC))-Based Materials.

Journal: Polymers
Published Date: Aug 26, 2018

Abstract

Poly([2-(methacryloyloxy)ethyl]trimethyl ammonium chloride) (METAC) and the gels were prepared and evaluated for their bactericidal and fungicidal activities. The antimicrobial properties of poly(METAC) were tested against (), (), (), methicillin-susceptible (MSSA), methicillin-resistant (MRSA), (), and (). Moreover, the structural forms of the linear and cross-linked poly(METAC) were investigated for their influences on bacterial aggregation, precipitation, and cell-death. To our knowledge, this is the first report on the comparison of the antimicrobial properties of poly(METAC) and poly(METAC)-gels. The bactericidal and fungicidal activities were evaluated by determining minimum inhibitory concentrations (MICs), UV⁻Vis spectroscopy, and fluorescence and confocal microscopies. The MICs were found to be 123 (MSSA), 123 (MRSA), 123 (), 370 (), 123 (), 370 (), and 370 μg/mL (), as determined by broth dilution, and 370 (MSSA), 370 (MRSA), 370 (), 3300 (), 370 (), 1100 (), and >10,000 μg/mL (), as determined by paper disc diffusion (on solid medium). The poly(METAC)-gels achieved rapid adsorption/precipitation of bacteria via the cationic surface charge. Thus, these poly(METAC)-based polymers can potentially be used as antibacterial materials.

Authors

  • Toshiki Shiga
    Department of Materials and Life Science, Shizuoka Institute of Science and Technology, 2200-2 Toyosawa, Fukuroi, Shizuoka 437-8555, Japan. giants_421_10@yahoo.co.jp.
  • Hiromitsu Mori
    Department of Materials and Life Science, Shizuoka Institute of Science and Technology, 2200-2 Toyosawa, Fukuroi, Shizuoka 437-8555, Japan. 1822008.mh@sist.ac.jp.
  • Keiichi Uemura
    Chutoen-General Medical Center, 1-1 Shobugaike, Kakegawa, Shizuoka 436-8555, Japan. k.uemura@fsinet.or.jp.
  • Ryota Moriuchi
    Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City, Shizuoka 422-8529, Japan. moriuchi.ryota@shizuoka.ac.jp.
  • Hideo Dohra
    Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City, Shizuoka 422-8529, Japan. dora.hideo@shizuoka.ac.jp.
  • Aika Yamawaki-Ogata
    Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan. aika@med.nagoya-u.ac.jp.
  • Yuji Narita
    Department of Cardiac Surgery, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan. ynarita@med.nagoya-u.ac.jp.
  • Akihiro Saito
    Department of Materials and Life Science, Shizuoka Institute of Science and Technology, 2200-2 Toyosawa, Fukuroi, Shizuoka 437-8555, Japan. saito.akihiro@sist.ac.jp.
  • Yohei Kotsuchibashi
    Department of Materials and Life Science, Shizuoka Institute of Science and Technology, 2200-2 Toyosawa, Fukuroi, Shizuoka 437-8555, Japan. kotsuchibashi.yohei@sist.ac.jp.

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