Molecular detection of in captive black-faced black spider monkeys (mammalia; primate).

Journal: Frontiers in veterinary science
Published Date: Jan 15, 2025

Abstract

The present study aimed to assess spp. infection in 17 captive black-faced black spider monkeys (), living at the Zoological Garden of Curitiba, Paraná, Brazil. Blood samples (3 mL) were collected by femoral puncture by certified veterinarians. A total of 5 μL of extracted blood DNA was submitted to Nested-PCR 18S rDNA. In overall, 4/17 (23.5%) spider monkeys were positive to spp. by PCR. Sequencing results for 18S rDNA revealed infection. This is the first report of in black-faced black spider monkey worldwide and the first assessment of species in NHP of southern Brazil.

Authors

  • Louise Bach Kmetiuk
    Graduate Program in Biosciences and Biotechnology, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, Paraná, Brazil.
  • Ana Silvia Miranda Passerino
    Curitiba City Secretary of Environment, Curitiba, Paraná, Brazil.
  • Marcelo Bonat
    Curitiba City Secretary of Environment, Curitiba, Paraná, Brazil.
  • Vívien Midori Morikawa
    Curitiba City Secretary of Environment, Curitiba, Paraná, Brazil.
  • Alice Pereira Berbigier
    Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil.
  • André Luiz Rodrigues Roque
    Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil.
  • Fabiano Borges Figueiredo
    Graduate Program in Biosciences and Biotechnology, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, Paraná, Brazil.
  • Alexander Welker Biondo
    Department of Veterinary Medicine, Federal University of Paraná, Curitiba, Paraná, Brazil.

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